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- Title
Interleukin-6 levels in female rats with protamine sulfate-induced chronic cystitis treated with hyaluronic acid.
- Authors
Lv, Yi‐Song; Yao, You‐Sheng; Lin, Ming‐En; Rong, Lu; Deng, Bi‐Hua; Huang, Jian; Hao, Wei‐Ping
- Abstract
Objectives To measure interleukin-6 levels in a protamine sulfate-induced chronic cystitis rat model treated with hyaluronic acid, and to study the correlation among interleukin-6, bladder inflammatory degree and voiding frequency. Methods A chronic cystitis model was created in female rats by using long-term intermittent intravesical protamine sulfate (0.5 mL, 30 mg/mL). Then, hyaluronic acid (0.5 mL, 0.8 mg/mL) was also instilled intravesically in the rats. Interleukin-6 levels were analyzed with immunohistochemistry, real-time reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Hematoxylin-eosin staining was carried out to examine bladder inflammatory degree based on a four-point scoring system (from 0 - none to 3 - severe). Voiding patterns were investigated by cystometrography. Results According to cystometrography, protamine sulfate-induced rats had significantly shorter intercontraction intervals and less bladder capacity ( P < 0.001). The bladder tissue of the rats showed severe chronic inflammation. Immunohistochemistry, reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay showed significantly higher expression of interleukin-6 ( P < 0.001). After intravesical administration of hyaluronic acid, both intercontraction intervals and bladder capacity increased significantly ( P < 0.001), whereas both bladder inflammatory degree and interleukin-6 levels decreased significantly ( P < 0.001). Furthermore, there was a strong correlation between interleukin-6 levels and inflammatory degree ( r = 0.727, P < 0.001), and also between interleukin-6 levels and voiding frequency ( r = -0.761, P < 0.001). Conclusions Intravesical administration of hyaluronic acid decreases interleukin-6 levels, as well as the severity of bladder inflammation and voiding frequency in a rat model of chronic cystitis. Interleukin-6 levels closely correlate with the inflammatory degree and voiding frequency. Thus, they can be regarded as an assessment measure of therapeutic impact.
- Subjects
INTERLEUKIN-6; PROTAMINES; CYSTITIS; HYALURONIC acid; IMMUNOHISTOCHEMISTRY
- Publication
International Journal of Urology, 2013, Vol 20, Issue 10, p1017
- ISSN
0919-8172
- Publication type
Article
- DOI
10.1111/iju.12090