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- Title
First report of genotype #65 of Toxoplasma gondii in pigs.
- Authors
Samico-Fernandes, Erika; de Melo, Renata; de Cássia Peixoto Kim, Pomy; de Almeida, Jonatas; de Barros, Luiz; Garcia, João; da Silva, Jean; Mota, Rinaldo
- Abstract
The aim of the present study was to isolate and genotype Toxoplasma gondii from pigs slaughtered for human consumption in northeastern Brazil. Indirect immunofluorescence antibody test (IFAT) was used to screen positive pigs. Tissues samples of animals with antibody titers ≥64 were submitted to bioassay in mice. One isolate of T. gondii was obtained, and the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique, using 11 markers (SAG1, SAG2, altSAG2, SAG3, BTUB, GRA6, c228, c292, L358, PK1, and APICO), was applied to evaluate the genetic variability. DNA from reference strains was used as a positive control. By means of genetic analysis, genotype ToxoDB #65 was identified, which is considered an atypical strain. This is the first record of genotype #65 in pigs. Thus, further studies in this region are necessary to determine the genetic variability of T. gondii in pigs and possible impact on public health.
- Subjects
TOXOPLASMA gondii; SWINE physiology; POLYMERASE chain reaction; GENOTYPES; RESTRICTION fragment length polymorphisms; PUBLIC health; GENETIC markers
- Publication
Parasitology Research, 2015, Vol 114, Issue 10, p3927
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-015-4664-z