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- Title
Characterization and vaccination of two novel Schistosoma japonicum genes screened from a cercaria cDNA library.
- Authors
Tian, Zhi; Wang, Shi-ping; Zeng, Shao-hua; Liu, Xue-qin; Gao, Dong-mei; Feng, Qi-mei; Zhang, Shu-ju; Zhou, Yun-fei; Wang, Xi-ya; Xia, Ying-ding
- Abstract
Two novel genes, SJCWL05 and SJCWL06, were harvested from screening of Schistosoma japonicum ( S. japonicum) cercaria cDNA library by using pig sera vaccinated (VPS) with S. japonicum immature egg ws-vaccine ( S. japonicum iEw). Prokaryotic recombinant plasmids pGEX-4T-1/SJCWL05 and pGEX-4T-1/SJCWL06 were constructed to analyze their immunogenicity, which was confirmed by SDS-PAGE and Western blotting. Two eukaryotic recombinant plasmids, pcDNA3/SJCWL05 and pcDNA3/SJCWL06, were constructed, and their ability to protect mice against challenge of S. japonicum was evaluated. All mice vaccinated with pcDNA3/SJCWL05 or pcDNA3/SJCWL06 developed ELISA-specific anti- S. japonicum SIEA ( S. japonicum soluble immature egg antigens) antibody. Immunoprotection experiments showed that worms and liver eggs reduced 34.64% and 39.14% in the pcDNA3/SJCWL05 group and those reduced 27.17% and 27.95% in the pcDNA3/SJCWL06 group, respectively. The reduction rates of intestine and uterine eggs in female worms of both groups reached 39.45% and 38.5% as well as 30.02% and 28.7%, respectively. Results of our study suggest that novel genes, SJCWL05 and SJCWL06, are potential vaccine candidates against schistosomiasis japonica.
- Subjects
CHINA; SCHISTOSOMIASIS; PUBLIC health; DRUG resistance; PLASMIDS; WESTERN immunoblotting; ENZYME-linked immunosorbent assay
- Publication
Parasitology Research, 2012, Vol 110, Issue 1, p403
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-011-2505-2