We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Genetic variation in Opisthorchis viverrini (Trematoda: Opisthorchiidae) from northeast Thailand and Laos PDR based on random amplified polymorphic DNA analyses.
- Authors
Paiboon Sithithaworn; Chadaporn Nuchjungreed; Tuanchai Srisawangwong; Katsuhiko Ando; Trevor Petney; Neil Chilton; Ross Andrews
- Abstract
Abstract??Genetic variation inOpisthorchis viverriniadults originating from different locations in northeast Thailand and Laos, People?s Democratic Republic (PDR), was examined using random amplified polymorphic DNA (RAPD) analyses. In an initial analysis, the genomic DNA of one fluke from each of ten localities was amplified using 15 random primers (10-mers); however, genetic variation amongO. viverrinispecimens was detected reliably for only four primers. A more detailed RAPD analysis using these four primers was conducted on ten individuals from nine localities. Considerable genetic variation was detected amongO. viverrinifrom different geographical areas and among some individuals from the same collecting locality. Comparison of the RAPD profiles revealed thatO. viverriniadults from Laos PDR were genetically distinct from those from northeast Thailand. The taxonomic significance of this finding needs to be explored in more detail. The RAPD markers established in the present study provide opportunities to examine the biology and epidemiology ofO. viverriniand fish-borne trematodes within the region. Additionally, application of these markers in such studies could have important implications in relation to the prevalence of cholangiocarcinoma in different regions of Asia.
- Subjects
THAILAND; LAOS; TREMATODA; RAPD technique
- Publication
Parasitology Research, 2007, Vol 100, Issue 3, p613
- ISSN
0932-0113
- Publication type
Article