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- Title
Identification of phenolic compounds from pollen extracts using capillary electrophoresis–electrospray time-of-flight mass spectrometry.
- Authors
Arráez-Román, D.; Zurek, G.; Bäßmann, C.; Almaraz-Abarca, N.; Quirantes, R.; Segura-Carretero, A.; Fernández-Gutiérrez, A.
- Abstract
In this work, a new, easy and rapid method of analyzing phenolic compounds in pollen extract, based on capillary electrophoresis coupled with electrospray ionization time-of-flight-mass spectrometry (CE–ESI–TOF–MS), has been developed. A systematic investigation of separation parameters has been performed with respect to resolution, sensitivity, analysis time and peak shape. The electrophoretic parameters and electrospray conditions must be optimized to obtain reproducible analyses. Using this method, several important phenolic compounds such as acetin-glucoside, 7-O-methylherbacetin-3-sophoroside, galloyl-glucose, quercetin-3-sophoroside, apigenin-6,8-di-C-glycoside, quercetin-3-rutinoside, genistein-7-O-β- D-glucoside, luteolin-7-O-glucoside, apigenin-7-O-glucoside and 2′,4′,6′-trihydroxy-3′-formyldihydrochalcone have been determined directly from pollen extract. The efficiency, the rapidity, the small amounts of sample required, and the high resolution of CE coupled with the sensitivity, the selectivity, the accurate masses and the true isotopic patterns obtained using TOF-MS point to the potential of this approach for identifying the phenolic compounds present in pollen.
- Subjects
PHENOLS; POLLEN extract; CAPILLARY electrophoresis; ELECTROSPRAY ionization mass spectrometry; BIOFLAVONOIDS; GLUCOSIDES; MASS spectrometry
- Publication
Analytical & Bioanalytical Chemistry, 2007, Vol 389, Issue 6, p1909
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-007-1611-6