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- Title
A simple monophasic LGPY medium for routine maintenance of Leishmania donovani promastigotes.
- Authors
Abhishek, Suman; Chaturvedi, Sanjay Kumar; Gupta, Anil Kumar; Ahmed, Ghufran; Das, Vidyanand Ravi; Narayan, Shyam
- Abstract
Here, we report a simple, economic and autoclavable monophasic LGPY medium supplemented with 10% fetal bovine serum (FBS), for routine maintenance of Leishmania donovani promastigotes for laboratory use. In comparison to commercially available M199 and RPMI-1640 media, LGPY has shown approximately seven fold more cell growth. The parasite has been observed to survive in the medium for at least 15 days post-inoculation. The medium also supports long-term sub-passaging of the promastigotes and can also be stored at 4 °C or room temperature for 14 months and 45 days, respectively.
- Subjects
LEISHMANIASIS; CELL growth; LEISHMANIA donovani; LEISHMANIA mexicana; BOS
- Publication
Parasitology Research, 2021, Vol 120, Issue 8, p2969
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-021-07193-w