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- Title
Developing a cell suspension system for Musa-AAA-EA cv. 'Nakyetengu': a critical step for genetic improvement of Matooke East African Highland bananas.
- Authors
Namanya, Priver; Mutumba, G.; Magambo, S.; Tushemereirwe, W.
- Abstract
Embryogenic cell suspensions of triploid East African Highland bananas (Musa AAA-EA) were initiated and generated using cooking cultivar 'Nakyetengu' belonging to the Nakabululu clone set. Immature male flowers produced embryogenic calli consisting of embryos and friable tissue after 4 mo culture on a modified MA1 callus induction medium. Friable calli were initiated and maintained in liquid MA2 medium. A cell growth rate of 1.5-2.0 sedimented cell volume (SCV) per month was observed. Embryo development was observed at 2.18 × 10 embryos per mL SCV. Germination of these embryos was observed at 2.8% and 6.2% for two cell suspension lines. Plant regeneration efficiency was 60-100%, all producing normal plants with a shoot and roots at weaning. In the field, somatic cell-derived plants were all normal morphology and comparable to control plants during vegetative and reproductive stages. This study is a breakthrough for recalcitrant East African Highland banana and offers a system that can provide essential raw materials for associated germplasm improvement through genetic engineering approaches.
- Subjects
EAST Africa; CELL suspensions; PLANT growing media; REGENERATION (Botany); PLANT germplasm; PLANT genetic engineering; PLANT embryology; BANANAS
- Publication
In Vitro Cellular & Developmental Biology Plant, 2014, Vol 50, Issue 4, p442
- ISSN
1054-5476
- Publication type
Article
- DOI
10.1007/s11627-014-9598-0