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- Title
甜菜 BvBADH 基因家族全基因组鉴定及其高盐胁迫下 的表达分析.
- Authors
李昊; 伍国强; 魏明; 韩悦欣
- Abstract
[Objective] Betaine aldehyde dehydrogenase (BADH) is one of the key enzymes involved in glycine betaine synthesis and plays an important role in plant response to abiotic stress. [Method] To explore the biological function and gene expression pattern of BvBADH gene family members in sugar beet (Beta vulgaris), the physicochemical properties of protein, phylogenetic evolution, gene structure, chromosome localization, conserved motif, protein structure, and promoter cis-acting regulatory elements of BvBADHs were analyzed by bioinformatics method, and their expression patterns under salt stress were investigated by RT-qPCR. [Result] A total of 9 BvBADH gene family members were identified. They contained 9-15 exons and 8-14 introns, the average amino acid number was 516, the average molecular weight was 55.84 kD, and the isoelectric point was 5.24-6.98. Phylogenetic analysis found that BADHs of higher plants were divided into three clusters, namely Clusters I, II, and III, among which Cluster III members can be further divided into two subgroups, including Cluster IIIa and IIIb. The members of BvBADH gene family were distributed in 3 clusters, respectively containing 3, 1, and 5 members. The promoter of the BvBADH gene mainly contained stress response elements, hormone response elements and growth and development response elements, the cis-acting regulatory element numbers of each member were 13-21. Further analysis of the expression pattern of the BvBADH genes in the sugar beet leaves showed that both 100 and 150 mmol/L NaCl significantly increased the expressions of all members of BvBADHs, but their expressions under 100 mmol/L NaCl were higher than those under 150 mmol/L NaCl. [Conclusion] The BvBADH gene family plays an important role in sugar beet's response to salt stress, it provides excellent genetic resources for the genetic improvement of crop stress tolerance in northern China.
- Publication
Biotechnology Bulletin, 2024, Vol 40, Issue 2, p233
- ISSN
1002-5464
- Publication type
Article
- DOI
10.13560/j.cnki.biotech.bull.1985.2023-0874