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- Title
Identification of a KIR antisense lncRNA expressed by progenitor cells.
- Authors
Wright, P W; Huehn, A; Cichocki, F; Li, H; Sharma, N; Dang, H; Lenvik, T R; Woll, P; Kaufman, D; Miller, J S; Anderson, S K
- Abstract
Human NK cells express cell surface class I MHC receptors (killer cell immunoglobulin-like receptor, KIR) in a probabilistic manner. Previous studies have shown that a distal promoter acts in conjunction with a proximal bidirectional promoter to control the selective activation of KIR genes. We report here the presence of an intron 2 promoter in several KIR genes that produce a spliced antisense transcript. This long noncoding RNA (lncRNA) transcript contains antisense sequence complementary to KIR-coding exons 1 and 2 as well as the proximal promoter region of the KIR genes. The antisense promoter contains myeloid zinc finger 1 (MZF-1)-binding sites, a transcription factor found in hematopoietic progenitors and myeloid precursors. The KIR antisense lncRNA was detected only in progenitor cells or pluripotent cell lines, suggesting a function that is specific for stem cells. Overexpression of MZF-1 in developing NK cells led to decreased KIR expression, consistent with a role for the KIR antisense lncRNA in silencing KIR gene expression early in development.
- Subjects
NON-coding RNA; PROGENITOR cells; GENE expression; IMMUNOGLOBULIN receptors; MAJOR histocompatibility complex; KILLER cells; RNA splicing
- Publication
Genes & Immunity, 2013, Vol 14, Issue 7, p427
- ISSN
1466-4879
- Publication type
Article
- DOI
10.1038/gene.2013.36