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- Title
The Cytotoxicity Assays of Hypoxia Mimicking Agent on Rabbit's (Oryctolagus cuniculus) Gingival Mesenchymal Stem Cells: Experimental In Vitro Study.
- Authors
NUGRAHA, ALEXANDER PATERA; IHSAN, IGO SYAIFUL; ANSORI, ARIF NUR MUHAMMAD; SUSILO, RADEN JOKO KUNCORONINGRAT; SUSILOWATI, HELEN; NARMADA, IDA BAGUS; ERNAWATI, DIAH SAVITRI; RANTAM, FEDIK ABDUL
- Abstract
Introduction: Cobalt (II) chloride hexahydrate (CoCl2) is a material that frequently used as hypoxia mimicking agent (HMA) to induce hypoxia condition in the cell culture. CoCl2 may stimulate the adaptive state and maintain the stemness of mesenchymal stem cells (MSCs). Even though CoCl2 commonly administered for cell culture, the biocompabilty of this material should be examined before use on Gingival Mesenchymal Stem Cells (GMSCs). Purpose: the purpose of this study is to examine the biocompability of CoCl2 as HMA on Rabbit's (Oryctolagus cuniculus GMSCs by using cytotoxicity assay. Materials and Methods: Rabbit's GMSCs at fourth subculture obtained from cell stock was used in this study. CoCl2 with the concentration as much as 200 θM, 150 θM, 100 θM, 75 θM, 50 θM, 25θM was used to be compared in this study. Cytotoxicity test of CoCl2 on GMSCs was conducted by means of 3-(4,5-Dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay. The formazan crystal was observed in the viable cells. The data were analyzed using one-way Analysis of Variance (ANOVA) with p<0.05 considered as significant. Results: The formazan crystals were found in the GMSCs co-cultured with various doses of CoCl2. There was no significant difference in the cell viability percentage of GMSCs between groups treated with CoCl2 with various concentration (p>0.05). Conclusion: CoCl2 as hypoxia mimicking agent are biocompatible to be used on rabbit's (O. cuniculus) gingival mesenchymal stem cells.
- Subjects
MESENCHYMAL stem cells; EUROPEAN rabbit; GINGIVA; HYPOXEMIA; ONE-way analysis of variance; CELL culture
- Publication
International Journal of Pharmaceutical Research (09752366), 2021, Vol 13, Issue 1, p4119
- ISSN
0975-2366
- Publication type
Article
- DOI
10.31838/ijpr/2021.13.01.545