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- Title
Immunogenicity and efficacy of a DNA vaccine in aged mice.
- Authors
Klinman, D M; Conover, J; Bloom, E T; Weiss, W
- Abstract
The immunogenicity and protective efficacy of a DNA vaccine encoding the circumsporozoite protein of the Plasmodium yoelii malaria parasite was evaluated in young (2 months) versus aged (>26 months) BALB/c mice. The primary and secondary humoral immune response of aged mice was 19- and 7-fold lower, respectively, than that of similarly treated young animals (p < .01). Cytotoxic T lymphocyte activity in aged mice was also lower than in younger animals. The vaccine response of aged animals was characterized by a 6-fold increase in interleukin-4 and a 3-fold increase in interferon-gamma (IFN-gamma) secreting cells, whereas in young animals immunization only stimulated the production of the type 1 cytokine IFN-gamma. Overall, 80% of young vaccinated mice were protected from subsequent challenge with live malaria sporozoites whereas only 40% of aged mice were protected. These results are the first to demonstrate that DNA vaccination induces less effective immunity in aged than young animals.
- Subjects
AGE distribution; AGING; ANALYSIS of variance; ANIMAL experimentation; COMPARATIVE studies; ENZYME-linked immunosorbent assay; RESEARCH methodology; MEDICAL cooperation; MICE; PROTEINS; PROTOZOA; RESEARCH; T cells; VACCINES; EVALUATION research
- Publication
Journals of Gerontology Series A: Biological Sciences & Medical Sciences, 1998, Vol 53A, Issue 4, pB281
- ISSN
1079-5006
- Publication type
journal article
- DOI
10.1093/gerona/53a.4.b281