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- Title
Differences in the Abundance of Auxin Homeostasis Proteins Suggest Their Central Roles for In Vitro Tissue Differentiation in Coffea arabica.
- Authors
Quintana-Escobar, Ana O.; Méndez-Hernández, Hugo A.; Galaz-Ávalos, Rosa M.; Elizalde-Contreras, José M.; Reyes-Soria, Francisco A.; Aguilar-Hernández, Victor; Ruíz-May, Eliel; Loyola-Vargas, Víctor M.
- Abstract
Coffea arabica is one of the most important crops worldwide. In vitro culture is an alternative for achieving Coffea regeneration, propagation, conservation, genetic improvement, and genome editing. The aim of this work was to identify proteins involved in auxin homeostasis by isobaric tandem mass tag (TMT) and the synchronous precursor selection (SPS)-based MS3 technology on the Orbitrap Fusion™ Tribrid mass spectrometer™ in three types of biological materials corresponding to C. arabica: plantlet leaves, calli, and suspension cultures. Proteins included in the β-oxidation of indole butyric acid and in the signaling, transport, and conjugation of indole-3-acetic acid were identified, such as the indole butyric response (IBR), the auxin binding protein (ABP), the ATP-binding cassette transporters (ABC), the Gretchen-Hagen 3 proteins (GH3), and the indole-3-acetic-leucine-resistant proteins (ILR). A more significant accumulation of proteins involved in auxin homeostasis was found in the suspension cultures vs. the plantlet, followed by callus vs. plantlet and suspension culture vs. callus, suggesting important roles of these proteins in the cell differentiation process.
- Subjects
COFFEE; TISSUE differentiation; ATP-binding cassette transporters; AUXIN; CARRIER proteins; CELL suspensions
- Publication
Plants (2223-7747), 2021, Vol 10, Issue 12, p2607
- ISSN
2223-7747
- Publication type
Article
- DOI
10.3390/plants10122607