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- Title
拟环纹豹蛛热休克蛋白20基因的克隆与分析.
- Authors
李长春; 王永; 姚国新; 戴余军; 李国元
- Abstract
【Objective】In order to provide some references for revealing the mechanisms by which spiders resisted to heavy metals,the small heat shock protein 20 gene(HSP20)of Pardosa pseudoannulata was cloned and its expression under cadmium was analyzed.【Method】Female P. pseudoannulata was used as materials,HSP20 gene of the P. pseudoannulata( PpHSP20)was cloned using RT-PCR technique based on the data obtained from transcriptome sequencing. PpHSP20 sequence characters were analyzed by bioinformatics method. The relative expression levels of PpHSP20 under cadmium stress were investigated by fluorescence quantitative PCR.【Result】The encoding region of the HSP20 gene was 525 bp in length,encoding 174 amino acids. Its molecular weight of encoded protein was 20.08 kD. It had 5.97 theoretical isoelectric point and typical α - crystallin domain of small heat shock proteinfamily. The deduced amino acid sequence of PpHSP20 shared high homology with α-crystallin of Parasteatoda tepidariorum(58%). Fluorescence quantitative PCR indicated that the expression of gene PpHSP20 under cadium stress increased significantly(P<0.05).【Conclusion】Gene PpHSP20 plays a regulation role in resistance to cadium stress,and can be an important candidate gene in research of spiders resisting cadium stress and a potential marker.
- Publication
Journal of Southern Agriculture, 2018, Vol 49, Issue 8, p1525
- ISSN
2095-1191
- Publication type
Article
- DOI
10.3969/j.issn.2095-1191.2018.08.09