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- Title
Differential effects of myosin light chain kinase inhibition on contractility, force development and myosin light chain 20 phosphorylation of rat cervical and thoracic duct lymphatics.
- Authors
Nepiyushchikh, Zhanna V.; Chakraborty, Sanjukta; Wang, Wei; Davis, Michael J.; Zawieja, David C.; Muthuchamy, Mariappan
- Abstract
Non-Technical Summary The contractile activities of lymphatic muscle cells, which are the key for the lymphatic function, vary between lymphatic beds. We show that pressure-dependent changes and myosin light chain kinase (MLCK) inhibition differentially affect myosin light chain 20 (MLC20) phosphorylation in lymphatic muscle, thereby controlling the contractile behaviour of thoracic duct and cervical lymphatics. Our findings suggest that pressure-induced changes in lymphatic contractile function act through MLC20 di-phosphorylation to modulate lymphatic tonic contractions, whereas the MLCK inhibitor ML-7 affects both mono- and di-phosphorylation of MLC20 and consequently decreases tonic contractions. In addition, our data indicate that ML-7 affects pacemaking activity of lymphatic muscle, and thereby decreases the lymphatic phasic contraction frequency. Thus the statuses of MLC20 phosphorylation play essential roles in regulating the contractile activities of lymphatics.
- Subjects
MYOSIN; MUSCLE cells; IMMUNE system; CHEMICAL inhibitors; PHOSPHORYLATION; LYMPHATICS
- Publication
Journal of Physiology, 2011, Vol 589, Issue 22, p5415
- ISSN
0022-3751
- Publication type
Article
- DOI
10.1113/jphysiol.2011.218446