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- Title
SILICON DEPOSITION DURING THE CELL CYCLE OF <em>THALASSIOSIRA WEISSFLOGH</em> (BACILLARIOPHYCEAE) DETERMINED USING DUAL RHODAMINE 123 AND PROPIDIUM IODIDE STAINING.
- Authors
Brzezinski, Mark A.; Conley, Daniel J.
- Abstract
The relatively non-toxic dye, rhodamine 123 (R123), was incorporated into the frustule of Thalassiosira weissflogii Grun. clone ACTIN in direct proportion to biogenic silica (BSi). R123 was used together with the DNA stain propidium iodide to track and quantify Si deposition during the cell cycle of T. weissflogii using flow cytometry, Silicon deposition was not continuous through the cell cycle. Deposition of the valves occurred during M phase. The hypocingulum was largely deposited during G 1 with some suggestion of minor or girdle band deposition during G2. Silicon deposition did not occur during S phase. Assuming that a complete pustule consists of an epivalve, epicingulum, hypocingulum, and hypavalve, then 40% of cellular BSi was contained within the cingulum of T. weissflogii with 60% present in the valves. These percentages correspond to 0.38 pmol Si in the two cingula and 0.57 pmol Si in the valves. Temporal differences in the timing of silicic acid uptake and deposition during the cell cycle of T. weissflogii suggested that deposition of both the new valves and the cingulum is supported by an internal pool of dissolved Si acquired during G2.
- Subjects
DIATOMS; BIOMINERALIZATION; SILICON; THALASSIOSIRA
- Publication
Journal of Phycology, 1994, Vol 30, Issue 1, p45
- ISSN
0022-3646
- Publication type
Article
- DOI
10.1111/j.0022-3646.1994.00045.x