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- Title
POLYCLONAL ANTIBODIES AGAINST IRON-SUPEROXIDE DISMUTASE FROM <em>ESCHERICHIA COLI</em> B CROSS-REACT WITH SUPEROXIDE DISMUTASES FROM <em>SYMIODINIUM MICROADRIATICUM</em> (DINOPHYCEAE).
- Authors
Matta, Jaime L.; Govind, Nadathur S.; Trench, Robert K.
- Abstract
Assays for superoxide dismutases (SODs) were performed using cell-free extracts of the symbiotic dinoflagellate Symbiodinium microadriaticm. Freudenthal (emend Trench and Blank) after separatioan in undenatured polyacrylamide gels. Using appropriate inhibitors (KCN and H[Sub2]O[Sub2]) we detected the presence of Cu/Zn[sup-1], Mn[Sup-], and Fe-SOD from Escherichia coli B crossreacted with two major polypeptides in the water-soluble fraction and one polypeptide in the Triton X-100-solubilized pellet fraction. The polypeptide common to both fractions, with a relative molecular mass of 43.5Da, was identified as Mn-SOD. In S.microadriatiacum, Fe-SOD, found only in the water-soluble fraction, appears to be monomeric,with a relative molecular mass of 49.5kDa.
- Subjects
ESCHERICHIA; DINOFLAGELLATES; IMMUNOGLOBULINS; ALGAE culture; PHYCOLOGY; BOTANY
- Publication
Journal of Phycology, 1992, Vol 28, Issue 3, p343
- ISSN
0022-3646
- Publication type
Article
- DOI
10.1111/j.0022-3646.1992.00343.x