We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Enhanced productions of poly-3-hydroxybutyrate and glycogen in cyanobacterium Synechocystis sp. PCC 6803 by disrupting related biosynthetic pathways under phosphorus deprivation.
- Authors
Kaewbai-ngam, Janine; Tanakarn Monshupanee
- Abstract
Under normal growth (NORMAL) condition with adequate supply of phosphorus and nitrogen, the wellstudied cyanobacterium Synechocystis wild type (WT) produced a high biomass level but subtle contents of the two biopolymers: poly-3-hydroxybutyrate (PHB) and glycogen. In contrast, under nitrogen deprivation, growth of WT was terminated, while the accumulations of PHB and glycogen were increased. Thus, a strain and a cultivation strategy that simultaneously allows both biomass production and the increased storages of the two biopolymers are desirable. Here, under phosphorus deprivation (-P), WT grew and still produced biomass at 850 mg/l (accounting for 62% of the biomass level obtained under NORMAL cultivation) with slightly increased contents of PHB and glycogen at 0.5% and 5.5% (w/w DW), respectively. Under -P, the △G mutant with the inactivated glycogen synthesis produced biomass at 751 mg/l and enhanced PHB accumulation to 4.9% (w/w DW), corresponding to PHB production of 36 mg/l. The △HP mutant with the inactivation of both PHB and hydrogen-gas syntheses under -P produced biomass at 713 mg/l and increased glycogen storage to 14.6% (w/w DW), corresponding to glycogen production of 118 mg/l. The significantly reduced levels of both chlorophyll and phycobilisomes (the main photosynthetic protein complex) found in △G and △HP mutants under -P suggested that photosynthetic proteins were degraded into amino acids which might subsequently be used for PHB and glycogen syntheses. The results indicated that the metabolic engineering combined with -P cultivation concurrently allowed cell growth and increased the production of two biopolymers in the studied Synechocystis.
- Subjects
POLY-beta-hydroxybutyrate; POLYHYDROXYBUTYRATE; GLYCOGEN; SYNECHOCYSTIS; BIOMASS production; PHOSPHORUS; PHYCOBILISOMES
- Publication
ScienceAsia, 2024, Vol 50, Issue 1, p1
- ISSN
1513-1874
- Publication type
Article
- DOI
10.2306/scienceasia1513-1874.2024.024