We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Identification of Circulating miR-22-3p and miR-93-5p as Stable Endogenous Control in Tuberculosis Study.
- Authors
Korma, Workneh; Mihret, Adane; Tarekegn, Azeb; Chang, Yunhee; Hwang, Dasom; Tessema, Tesfaye Sisay; Lee, Hyeyoung
- Abstract
The diagnosis and prognosis of tuberculosis remains challenging and necessitates the development of a new test that can accurately diagnose and monitor treatment responses. In this regard, miRNA is becoming a potential diagnostic and prognostic biomarker which differentiates treatment respondents from non-respondents for various non-infectious and infectious diseases, including tuberculosis. The concentration of miRNAs varies based on cell type, disease, and site of infection, implicating that selection of an optimal reference gene is crucial, and determines the quantification of transcript level and biological interpretation of the data. Thus, the study evaluated the stability and expression level of five candidate miRNAs (let-7i-5p, let-7a-5p, miRNA-16-5p, miRNA-22-3p and miRNA-93-5p), including U6 Small Nuclear RNA (RNU6B) to normalize circulating miRNAs in the plasma of 68 participants (26 healthy controls, 23 latent, and 19 pulmonary tuberculosis infected) recruited from four health centers and three hospitals in Addis Ababa, Ethiopia. The expression levels of miRNAs isolated from plasma of culture confirmed newly diagnosed pulmonary tuberculosis patients were compared with latently infected and non-infected healthy controls. The qPCR data were analyzed using four independent statistical tools: Best Keeper, Genorm, Normfinder and comparative delta-Ct methods, and the data showed that miRNA-22-3p and miRNA-93-5p were suitable plasma reference miRNAs in a tuberculosis study.
- Subjects
ADDIS Ababa (Ethiopia); SMALL nuclear RNA; TUBERCULOSIS; NON-communicable diseases; COMMUNICABLE diseases; TUBERCULOSIS patients
- Publication
Diagnostics (2075-4418), 2020, Vol 10, Issue 11, p868
- ISSN
2075-4418
- Publication type
Article
- DOI
10.3390/diagnostics10110868