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- Title
Therapeutic Effect and Mechanism of Caulophyllum robustum Maxim Extract on Adjnvant Arthritis Rats.
- Authors
Shaowa LU; Hong SU; Fengming YU; Qiushi WANG; Yuyan GUO; Guoyu LI; Haixue KUANG
- Abstract
[Objectives] To study the anti-inflammation and anti-swelling effect of Caulophyllum robustum Maxim (CRM) extract on secondary lesions of adjuvat athritis (AA), associated inflammatory factors of AA rats ad mice immune function. [Methods] Rats were divided into high, middle ad low dose CRM extract groups (69.23, 34. 61, 17.31 mg/kg), methotrexte group (0.68 mg/kg), triptolide group (7.88 mg/kg), model goup and normal control group. The AA rat model was induced by intradermal injection of Freund's complete adjuvat (FCA). After the AA model was established successfully, the paw swelling of AA rats was observed and recorded. The levels of interleukin-Iβ (IL-1β), interleukin-(IL-4), interleukin-10 (IL-10), Tumor Necrosis Factor-α (TNF-α), interleukin 17 (IL-17), interferon (IFN-γ), granulocyte colony-stimulating factor (G-CSF) and transforming growth factor (TGF-β) in the serum of AA rats were detected by enzyme-linked immunosorbent assay (ELISA). The spleen was removed and weighed for detecting the drug influence on the immune organs. Impacts were observed by gavage administration at different dosages of 25 mg/kg, 50 mg/kg, 100 mgkg for seven days, adopting carbon granules clearance test, delayed-type hypersensitivity induced by dinitrofluorobenzene (DNFB) coupled with flow cytometry evaluating lymphocytes (CD3+, CD4+, CD8+) activation and serolysin test. [Results] Compared with the model goup, the joint swelling, spleen index and inflammatory cytokines (IL-1β, IL-4, IL-10, TNF-α, IL-17, IFN-γ, G-CSF, TGF-β) were reduced significantly (P<0.05, P<0.01). And the activity of CRM was significantly superior to methotrexate and triptergium. Moreover, the results indicated that different dosages of 25 mg/kg, 50 mg/kg, 100 mg/kg of CRM could significantly reduce the carbon granules clearance index (K) and phagocytic index (a), inhibit the phaocytosis of mononuclear macrophages, reduce the mice swelling extent ad inhibit DTH in mice induced by DNFB, decrease the quality of serum hemolysin. Percentage of CD3, CD4, and the ratio of CD4, CD8 notably decreased, whereas CD8 obviously increased. Intragastrica dose of 50 mg/kg, 100 mg/kg were acknowledged as more objective inhibiting effect compared with a dosage of 25 mg/kg. [Conclusions] Decreasing inflammatory cytokines content, non-specific immune, humoral immune ad cellular immune function may be the therapeutic mechanism for rheumatoid arthritis (RA).
- Subjects
MEDICINAL plants; ARTHRITIS; INFLAMMATION; ENZYME-linked immunosorbent assay; GRANULOCYTES
- Publication
Medicinal Plant, 2018, Vol 9, Issue 1, p50
- ISSN
2152-3924
- Publication type
Article
- DOI
10.19600/j.cnki.issn2152-3924.2018.01.013