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- Title
ExGen 500 is an efficient vector for gene delivery to lung epithelial cells in vitro and in vivo.
- Authors
Ferrari, S; Moro, E; Pettenazzo, A; Behr, J P; Zacchello, F; Scarpa, M
- Abstract
Nonviral vectors might represent a safe alternative to adenovirus for gene therapy of lung disorders, in particular cystic fibrosis (CF). Cationic lipids have been shown to correct the CF defect both in vitro and in vivo, but more efficient vectors are needed to improve the low gene transfer efficiency. Here, we show that the cationic polymer ExGen 500, a linear polyethylenimine derivative, is more efficient than cationic lipids in transferring reporter genes to lung epithelial cells in vitro. In vivo ExGen 500 was able to mediate gene transfer into both newborn and adult rabbit lungs with comparable efficiencies. The best levels of transfection were obtained using neutral complexes. Under such conditions, luciferase activities corresponding to about 103 RLU/10 s/mg of protein were reproducibly obtained 2 days after transfection throughout the four lung lobes of newborn and adult rabbits. A nlslacZ reporter gene showed transfected cells around the lumen of large and small bronchi. No signs of acute toxicity (inflammation, cellular infiltration etc) were detected by direct histopathological analysis. Within 1 week after instillation, transgene expression decreased by two orders of magnitude.
- Subjects
GENES; EPITHELIAL cells; LIPIDS
- Publication
Gene Therapy, 1997, Vol 4, Issue 10, p1100
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/sj.gt.3300503