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- Title
Sulfatide inhibits α-galactosylceramide presentation by dendritic cells.
- Authors
Kanamori, Mitsuhiro; Tasumi, Yusuke; Iyoda, Tomonori; Ushida, Maki; Inaba, Kayo
- Abstract
Sulfatide-reactive type II NKT cells, the so-called non-invariant NKT (non-iNKT) cells, have been shown to counteract invariant NKT (iNKT) cell activity. However, the effects of sulfatide on activation of iNKT cells by α-galactocylceramide (αGC) in the context of CD1d have not been studied in detail. Therefore, we studied the blocking effect of sulfatide on αGC-induced iNKT cell activation by dendritic cells (DCs). Even in the absence of non-iNKT cells, sulfatide inhibited αGC-mediated iNKT cell activation by reducing αGC/CD1d complex formations in a dose-dependent manner. This was also confirmed in a cell-free setting using immobilized CD1d-Ig. Moreover, simultaneous injection of αGC with sulfatide decreased αGC/CD1d complex formations on DCs, accompanied by the reduced CD40L-up-regulation and IFN-γ production by iNKT cells and IL-12p70 production by DCs. However, sulfatide by itself did not interfere with the presentation of MHC class II-mediated antigen presentation to specific T cells. These results demonstrate that sulfatide competes with αGC to be loaded onto CD1d along the endocytic pathway in DCs, thereby inhibiting the iNKT cell response.
- Subjects
SULFATIDES; DENDRITIC cells; KILLER cells; MAJOR histocompatibility complex; T cells; IMMUNE response
- Publication
International Immunology, 2012, Vol 24, Issue 2, p129
- ISSN
0953-8178
- Publication type
Article
- DOI
10.1093/intimm/dxr108