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- Title
E‐Cadherin restricts mast cell degranulation in mice.
- Authors
Klewer, Theres; Bakic, Ljubica; Müller‐Reichert, Thomas; Kiewisz, Robert; Jessberger, Gregor; Kiessling, Nadine; Roers, Axel; Jessberger, Rolf
- Abstract
Crosslinking of FcεRI‐bound IgE triggers the release of a large number of biologically active, potentially anaphylactic compounds by mast cells. FcεRI activation ought to be well‐controlled to restrict adverse activation. As mast cells are embedded in tissues, adhesion molecules may contribute to limiting premature activation. Here, we report that E‐Cadherin serves that purpose. Having confirmed that cultured mast cells express E‐Cadherin, a mast‐cell‐specific E‐Cadherin deficiency, Mcpt5‐Cre E‐Cdhfl/fl mice, was used to analyze mast cell degranulation in vitro and in vivo. Cultured peritoneal mast cells from Mcpt5‐Cre E‐Cdhfl/fl mice were normal with respect to many parameters but showed much‐enhanced degranulation in three independent assays. Soluble E‐Cadherin reduced the degranulation of control cells. The release of some newly synthesized inflammatory cytokines was decreased by E‐Cadherin deficiency. Compared to controls, Mcpt5‐Cre E‐Cdhfl/fl mice reacted much stronger to IgE‐dependent stimuli, developing anaphylactic shock. We suggest E‐Cadherin‐mediated tissue interactions restrict mast cell degranulation to prevent their precocious activation.
- Subjects
MAST cells; CADHERINS; ANAPHYLAXIS; MICE; IMMUNOGLOBULIN E
- Publication
European Journal of Immunology, 2022, Vol 52, Issue 1, p44
- ISSN
0014-2980
- Publication type
Article
- DOI
10.1002/eji.202049087