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- Title
On demand delivery and analysis of single molecules on a programmable nanopore-optofluidic device.
- Authors
Rahman, M.; Stott, M. A.; Harrington, M.; Li, Y.; Sampad, M. J. N.; Lancaster, L.; Yuzvinsky, T. D.; Noller, H. F.; Hawkins, A. R.; Schmidt, H.
- Abstract
Nanopore-based single nanoparticle detection has recently emerged as a vibrant research field with numerous high-impact applications. Here, we introduce a programmable optofluidic chip for nanopore-based particle analysis: feedback-controlled selective delivery of a desired number of biomolecules and integration of optical detection techniques on nanopore-selected particles. We demonstrate the feedback-controlled introduction of individual biomolecules, including 70S ribosomes, DNAs and proteins into a fluidic channel where the voltage across the nanopore is turned off after a user-defined number of single molecular insertions. Delivery rates of hundreds/min with programmable off-times of the pore are demonstrated using individual 70S ribosomes. We then use real-time analysis of the translocation signal for selective voltage gating of specific particles from a mixture, enabling selection of DNAs from a DNA-ribosome mixture. Furthermore, we report optical detection of nanopore-selected DNA molecules. These capabilities point the way towards a powerful research tool for high-throughput single-molecule analysis on a chip. Nanopore based detection has numerous high impact applications outside of next-generation sequencing. Here the authors use an optofluidic chip to detect individual individual 70S ribosomes in a sample mixed with DNA.
- Subjects
SINGLE molecules; OPTOFLUIDICS; NANOPORES; BIOMOLECULES; NUCLEOTIDE sequencing
- Publication
Nature Communications, 2019, Vol 10, Issue 1, pN.PAG
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-019-11723-7