We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
In vitro conservation of 'Florida Rough' lemon plants.
- Authors
da Silva de Carvalho, Mariane de Jesus; da Silva Souza, Antônio; Barbosa Santos, Emanuela; dos Santos Soares Filho, Walter; da Silva Ledo, Carlos Alberto; Rodrigues Costa, Eva Maria; Duarte Souza, Fernanda Vidigal
- Abstract
The establishment of minimum growth conditions is essential for in vitro germplasm conservation. Changes to the basic medium and carbon source concentrations are important factors for reducing plant growth in vitro. This study adjusted a protocol for the in vitro conservation of 'Florida Rough' lemon plants. Microcuttings (approximately 1 cm) from plants that were previously cultivated in vitro were inoculated into test tubes with 10 mL of woody plant medium (WPM) at different concentrations (1/1, 1/2 and 1/4) and supplemented with 0, 12.5, 25 and 50 g.L-1 of sucrose, solidified with 7 g.L-1 agar and adjusted to pH 5.8. The experiment was completely randomized in a 3 x 4 factorial design with 15 replications and was maintained under controlled conditions for 360 days. After this period the plant height in cm (PH), the plant dry mass in g (PDM) and the 21 numbers of green leaves (NGL), senescent leaves (NSL) and microcuttings (NM) were evaluated. The variables that best explained the observed behavior of the 'Florida Rough' lemon plants were NGL and PH, with values of 61.63 and 35.08%, respectively. The original concentration of the WPM with the addition of 25 g. L-1 of sucrose yielded the best 'Florida Rough' lemon plant growth reduction in vitro while maintaining the physiologically health of the plants.
- Subjects
GERMPLASM; GERMPLASM conservation; LEMON; WOODY plants; FACTORIAL experiment designs; PLANT drying
- Publication
Ciência Rural, 2022, Vol 52, Issue 12, p1
- ISSN
0103-8478
- Publication type
Article
- DOI
10.1590/0103-8478cr20210276