We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Comparative analysis of the root transcriptomes of cultivated and wild rice varieties in response to <italic>Magnaporthe oryzae</italic> infection revealed both common and species-specific pathogen responses.
- Authors
Tian, Lei; Shi, Shaohua; Nasir, Fahad; Chang, Chunling; Li, Weiqiang; Tran, Lam-Son Phan; Tian, Chunjie
- Abstract
Background: <italic>Magnaporthe oryzae</italic>, the causal fungus of rice blast disease, negatively impacts global rice production. Wild rice (<italic>Oryza rufipogon</italic>), a relative of cultivated rice (<italic>O. sativa</italic>), possesses unique attributes that enable it to resist pathogen invasion. Although wild rice represents a major resource for disease resistance, relative to current cultivated rice varieties, no prior studies have compared the immune and transcriptional responses in the roots of wild and cultivated rice to <italic>M. oryzae</italic>.Results: In this study, we showed that <italic>M. oryzae</italic> could act as a typical root-infecting pathogen in rice, in addition to its common infection of leaves, and wild rice roots were more resistant to <italic>M. oryzae</italic> than cultivated rice roots. Next, we compared the differential responses of wild and cultivated rice roots to <italic>M. oryzae</italic> using RNA-sequencing (RNA-seq) to unravel the molecular mechanisms underlying the enhanced resistance of the wild rice roots<italic>.</italic> Results indicated that both common and genotype-specific mechanisms exist in both wild and cultivated rice that are associated with resistance to <italic>M. oryzae.</italic> In wild rice, resistance mechanisms were associated with lipid metabolism, WRKY transcription factors, chitinase activities, jasmonic acid, ethylene, lignin, and phenylpropanoid and diterpenoid metabolism; while the pathogen responses in cultivated rice were mainly associated with phenylpropanoid, flavone and wax metabolism. Although modulations in primary metabolism and phenylpropanoid synthesis were common to both cultivated and wild rice, the modulation of secondary metabolism related to phenylpropanoid synthesis was associated with lignin synthesis in wild rice and flavone synthesis in cultivated rice. Interestingly, while the expression of fatty acid and starch metabolism-related genes was altered in both wild and cultivated rice in response to the pathogen, changes in lipid acid synthesis and lipid acid degradation were dominant in cultivated and wild rice, respectively.Conclusions: The response mechanisms to <italic>M. oryzae</italic> were more complex in wild rice than what was observed in cultivated rice. Therefore, this study may have practical implications for controlling <italic>M. oryzae</italic> in rice plantings and will provide useful information for incorporating and assessing disease resistance to <italic>M. oryzae</italic> in rice breeding programs.
- Subjects
RICE varieties; RICE farming; RICE blast disease; RED rice; RICE disease &; pest resistance
- Publication
Rice (19398425), 2018, Vol 11, Issue 1, p1
- ISSN
1939-8425
- Publication type
Article
- DOI
10.1186/s12284-018-0211-8