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- Title
Characterization of vB#&95Kpn#&95F48, a Newly Discovered Lytic Bacteriophage for Klebsiella pneumoniae of Sequence Type 101.
- Authors
Ciacci, Nagaia; D'Andrea, Marco Maria; Marmo, Pasquale; Demattè, Elisa; Amisano, Francesco; Di Pilato, Vincenzo; Fraziano, Maurizio; Lupetti, Pietro; Rossolini, Gian Maria; Thaller, Maria Cristina
- Abstract
Resistance to carbapenems in Enterobacteriaceae, including Klebsiella pneumoniae, represents a major clinical problem given the lack of effective alternative antibiotics. Bacteriophages could provide a valuable tool to control the dissemination of antibiotic resistant isolates, for the decolonization of colonized individuals and for treatment purposes. In this work, we have characterized a lytic bacteriophage, named vB_Kpn_F48, specific for K. pneumoniae isolates belonging to clonal group 101. Phage vB_Kpn_F48 was classified as a member of Myoviridae, order Caudovirales, on the basis of transmission electron microscopy analysis. Physiological characterization demonstrated that vB_Kpn_F48 showed a narrow host range, a short latent period, a low burst size and it is highly stable to both temperature and pH variations. High throughput sequencing and bioinformatics analysis revealed that the phage is characterized by a 171 Kb dsDNA genome that lacks genes undesirable for a therapeutic perspective such integrases, antibiotic resistance genes and toxin encoding genes. Phylogenetic analysis suggests that vB_Kpn_F48 is a T4-like bacteriophage which belongs to a novel genus within the Tevenvirinae subfamily, which we tentatively named "F48virus". Considering the narrow host range, the genomic features and overall physiological parameters phage vB_Kpn_F48 could be a promising candidate to be used alone or in cocktails for phage therapy applications.
- Subjects
CARBAPENEMASE; ENTEROBACTERIACEAE; KLEBSIELLA pneumoniae; MYOVIRIDAE; TRANSMISSION electron microscopy
- Publication
Viruses (1999-4915), 2018, Vol 10, Issue 9, p482
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v10090482