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- Title
The Escherichia coli ribosomal protein S16 is an endonuclease.
- Authors
Oberto, Jacques; Bonnefoy, Eliette; Mouray, Elisabeth; Pellegrini, Olivier; Wikstróm, P. Mikael; Rouvière-Yaniv, Josette
- Abstract
The histone-like protein HU isolated from <em>Escherichia Coli</em> exhibited, after several purification steps, a Mg2+-dependent nuclease activity. We show here that this activity can be dissociated from HU by a denaturation-renaturation step, and is due to a small fraction of ribosomal protein S16 co-purifying with HU. S16 is an essential component of the 30S ribosomal particles. We have cloned, overproduced, and purified a histidine-tagged S16 and shown that this protein is a DNA-binding protein carrying a Mgs2+-Mn2+-dependent endonuclease activity. This is an unexpected property for a ribosomal protein.
- Subjects
ENDONUCLEASES; ESCHERICHIA coli; CARRIER proteins; PROTEINS; NUCLEASES
- Publication
Molecular Microbiology, 1996, Vol 19, Issue 6, p1319
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.1996.tb02476.x