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- Title
Analysis of the rnc locus of Coxiella burnetii.
- Authors
Zuber, Mohammed; Hoover, Timothy A.; Powell, Bradford S.; Court, Donald L.
- Abstract
A 3.2kb <em>Eco</em>RI genomic DNA fragment of <em>Coxiella burnetii</em> was isolated by virtue of its ability to suppress mucoidy in <em>Escherichia coli</em>. Nucleotide sequence analysis revealed the presence of the genes homologous to <em>rnc, era</em> and <em>recO</em> of <em>E. coli</em>. Suppression of capsule synthesis, measured by β-galactosidase expression in <em>ion</em>- cps-lac fusion strains of <em>E. coli</em>, is caused by gene-dosage effects of the plasmid-borne <em>rnc</em> genes of either <em>C. burnetii</em> or <em>E. coli</em>. The <em>rnc</em> gene of <em>C. burnetii</em> complemented rnc- <em>E. coli</em> hosts for lembda plaque morphology and stimulation of lambda <em>N</em> gene expression. We also demonstrated heterologous complementation of an <em>E. coli</em> strain defective for the expression of Era, an essential protein in <em>E. coli</em>, using the plasmid-borne <em>C. burnetii era</em>. Under the control of the bacteriophage lambda PL promoter, this 3.2kb EcoRI DNA fragment directed the synthesis in <em>E. coli</em> of three proteins with approximate molecular masses of 35, 27 and 25 kDa. Antibodies against purified <em>E. coli</em> Era protein cross-reacted with the 35 kDa protein of <em>C. burnetii</em> on Western blots.
- Subjects
DNA; COXIELLA burnetii; NUCLEOTIDE analysis; GENE expression; ESCHERICHIA coli
- Publication
Molecular Microbiology, 1994, Vol 14, Issue 2, p291
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.1994.tb01290.x