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- Title
Plasma-based detection of clonality in lymphoid malignancies.
- Authors
Chen-Hsiung Yeh; Tseng, Richard; Albitar, Maher
- Abstract
Objectives: Plasma has been found to be enriched with tumor-specific DNA, RNA, and protein in patients with hematologic disease. We assessed the utility of plasma as a DNA source for detection of genetic abnormalities in patients with suspected B- or T-cell lymphoproliferative disorders. Methods: DNA was extracted from paired peripheral blood (PB) cells and plasma for polymerase chain reaction (PCR)-based detection of immunoglobulin heavy chain ( IgH) and T-cell receptor gamma chain ( TCR-γ) rearrangements, and B-cell leukemia/lymphoma ( BCL)- 1/IgH and BCL-2/ IgH translocations. Results: Concordance between plasma and PB cell analysis was 100% for IgH ( n = 57), TCR-γ ( n = 57), and BCL-1/ IgH ( n = 37) rearrangements, and 94% (60/64) for BCL-2/ IgH; four of 11 plasma samples positive for BCL-2/ IgH tested negative in paired cells. No plasma or PB cell samples from 195 healthy donors showed genetic abnormalities. Conclusions: These findings indicate that plasma is a reliable sample type for detection of abnormalities associated with B- and T-cell lymphoproliferative disorders, providing sensitivity equal to or greater than that of PB cells.
- Subjects
LYMPHOID tissue; CELL proliferation; CELL receptors; POLYMERASE chain reaction; RNA; DNA polymerases; BLOOD diseases
- Publication
European Journal of Haematology, 2009, Vol 82, Issue 6, p450
- ISSN
0902-4441
- Publication type
Article
- DOI
10.1111/j.1600-0609.2009.01231.x