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- Title
Toxoplasma gondii RPL40 is a circulating antigen with immune protection effect.
- Authors
Junxin Xue; Wei Jiang; Jian Li; Wei Xiong; Zhengan Tian; Qiang Zhang; Shuqing Li; Chunfeng Liu; Kehe Huang; Quan Wang
- Abstract
Screening and identification of protective antigens are essential for the prevention of infections with Toxoplasma gondii (Nicolle et Manceaux, 1908). In our previous study, T. gondii ribosomal-ubiquitin protein L40 (TgRPL40) was identified as a circulating antigen. However, the function and protective value of TgRPL40 was unknown. In the current study, recombinant TgRPL40 was expressed in Escherichia coli BL21 and antibody was prepared. Western blotting analysis indicated that TgRPL40 was present in circulating antigens and excretory/secretary antigens (ESA). Immunofluorescence and immunoelectron microscopy analysis revealed that TgRPL40 protein is widely distributed in the tachyzoites. Immunisation with recombinant TgRPL40 prolonged the survival of mice infected with tachyzoites. Quantitative real-time polymerase chain reaction analysis showed that immunisation with recombinant TgRPL40 reduced the parasite burden in blood, liver, spleen and brain of mice infected with tachyzoites. These observations indicate that TgRPL40 is a circulating antigen and is an effector of immune protection against acute T. gondii infection.
- Subjects
TOXOPLASMA gondii; PROTEIN expression; ESCHERICHIA coli; RECOMBINANT proteins; IMMUNOELECTRON microscopy; POLYMERASE chain reaction
- Publication
Folia Parasitologica, 2019, Vol 66, p1
- ISSN
0015-5683
- Publication type
Article
- DOI
10.14411/fp.2019.013