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- Title
Effects of Plant Growth Regulators on Haploid Production of Wheat.
- Authors
Young Jin Kim; Kwang Won Lee; Hag Sin Kim; Young Keun Cheong; Kwang Geun Park; Kee Jong Kim
- Abstract
The introduction of doubled haploid (DH) approach into breeding programs has reduced the times and population sizes required for the production of pure lines. We carried out the experiment for development on effective method of producing haploid in wheat. Emasculated spikelets of wheat were pollinated with maize pollen and cultured in the solution containing 40 g/L sucrose and 2,4-D, NAA, 2,4,5-T and dicamba 24 h after pollination, and then incubated until embryo rescue. Fourteen to sixteen days after pollination, the embryos are excised and cultured in half-strength MS basal medium supplemented with 20 g/L sucrose and 1 mg/L NAA. The type of plant growth regulators was found to be most significant in production of haploid plants. The application of synthetic auxins to pollinated florets, stimulates haploid embryo development to a stage where the embryos can be rescued onto nutrient media. The percentage of seeded florets was significantly affected by 100 mg/L 2,4-D, 150 mg/L 2,4,5-T and 50 mg/L dicamba. The percentage of embryos formed was significantly increased by treatment with 2,4-D and 2,4,5-T at 100 mg/L, and dicamba at 50 mg/L, but the treatments with 150 mg/L 2,4-D inhibited embryo development and plant regeneration. The optimum application time of plant growth regulators was 24 hrs after pollination.
- Subjects
WHEAT; PLANT regulators; HAPLOIDY; PLANT embryology; POLLINATION; PLANT breeding
- Publication
Korean Journal of Breeding Science, 2011, Vol 43, Issue 1, p50
- ISSN
0250-3360
- Publication type
Article