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- Title
Human Lymphoblastoid Cell Line Producing Specific Antibody against Rh-Antigen D.
- Authors
Koskimies, S.
- Abstract
A lymphoblastoid cell line producing specific anti-Rh antibody against D determinant was established by Epstein-Barr virus (EBV) infection. The lymphocytes were from an immunized male donor with high titre of anti-D antibody. The cells were preselected by rosetting them with Rh-positive erythrocytes before EBV infection. The cell line produced specific antibody of IgG class, namely IgG1 subclass, but it also produced nonspecific IgM and IgG. By rerosetting the cell line, the specific antibody-producing population could be enriched and the antibody titre increased in the culture supernatant to the level of immune sera.
- Subjects
LYMPHOBLASTOID cell lines; IMMUNOGLOBULIN G; LEUCOCYTES; CELL culture; IMMUNE serums; BLOOD plasma
- Publication
Scandinavian Journal of Immunology, 1980, Vol 11, Issue 1, p73
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1980.tb00210.x