We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
DNA Synthesis in Subpopulations of Blood Mononuclear Leucocytes in Human Subjects after Vaccination against Yellow Fever.
- Authors
Ehrnst, A.; Lambert, B.; Fagraeus, A.
- Abstract
After vaccination of five volunteers with yellow fever live vaccine, blood mononuclear cells were isolated and labelled with ³H-thymidine at intervals. DNA synthesis was measured by scintillation counting and autoradiography of rosetted cells. Rosetting with sheep erythrocytes (E-RFC) identified T cells, and such erythrocytes coated with IgM antibodies and complement (EAC-RFC) identified B cells and monocytes. DNA synthesis in the total mononuclear cell fraction, as well as in subfractions enriched in or deprived of E-RFC, displayed a sharp increase on day 10-11 after vaccination, remained high on day 13-14, and then returned to the prevaccination level, There was a corresponding morphological transformation, measured by size distribution and number of nucleoli per cell. The major fraction of DNA-synthesizing cells before, during and after the peak of activity was found among non-rosette-forming cells. However, during the activity peak the numbers and proportion of DNA-synthesizing E-RFC were increased while the response with regard to EAC-RFC was not obvious. Thus within a complex cellular response a transient T-cell response was identified.
- Subjects
LEUCOCYTES; YELLOW fever vaccines; DNA synthesis; LYMPHOCYTES; B cells; MONOCYTES
- Publication
Scandinavian Journal of Immunology, 1978, Vol 8, Issue 4, p339
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1978.tb00527.x