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- Title
The morphology and in vitro antioxidative effect of ionized polysaccharide from Angelica Sinensis.
- Authors
Jie Wang; Zizhen Huang; Weijiao Xue; Yan Zhang; Chunxia Tan
- Abstract
In the present study, the effect of ionization modification on the morphology and in vitro antioxidant activity of Angelica Sinensis polysaccharide (ASP) was researched. ASP was used as raw material to obtain phosphorylated Angelica Sinensis polysaccharide (ASP-P) and cationized Angelica Sinensis polysaccharide (ASP-N). The degree of phosphorylation and cationization of ASP were determined by experiments. Structural characterization and thermal stability testing of ionized ASP derivatives were explored by infrared spectroscopy, thermogravimetric analysis, X-red diffraction, dynamic light scattering, Congo-red test and scanning electron microscopy. The ionization modified ASP have better thermal stability, among which ASP-P has the best thermal stability. ASP-P and ASP-N showed more ordered structure in solid state and the ionized ASP-P and ASP-N in solution all maintain the triple helix architecture of ASP, but exhibited a larger size of stacked or curled sheet morphology in solidity. The scavenging assays of hydroxyl radical, ABTS and DPPH radical were used to evaluate the changes in the antioxidant capacity of ASP before and after ionization modification. The ionic modification products of ASP can significantly improve the scavenging ability in vitro. The above experimental results provide an experimental basis for further research on the derivatization of ASP.
- Subjects
DONG quai; HYDROXYL group; THERMAL stability; SCANNING electron microscopy; POLYSACCHARIDES; INFRARED spectroscopy
- Publication
Journal of Chinese Pharmaceutical Sciences, 2024, Vol 33, Issue 4, p316
- ISSN
1003-1057
- Publication type
Article
- DOI
10.5246/jcps.2024.04.024