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- Title
Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering.
- Authors
Wei, Lu; Hu, Fanghao; Shen, Yihui; Chen, Zhixing; Yu, Yong; Lin, Chih-Chun; Wang, Meng C; Min, Wei
- Abstract
Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic incorporation of alkyne-tagged small precursors.
- Subjects
BIOMOLECULES; RAMAN scattering; MOLECULAR biology; NUCLEIC acids; HUMAN genetics; ANIMAL cell biotechnology; LABORATORY mice
- Publication
Nature Methods, 2014, Vol 11, Issue 4, p410
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.2878