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- Title
Mapping genetic interactions in human cancer cells with RNAi and multiparametric phenotyping.
- Authors
Laufer, Christina; Fischer, Bernd; Billmann, Maximilian; Huber, Wolfgang; Boutros, Michael
- Abstract
Genetic interactions influence many phenotypes and can be used as a powerful experimental tool to discover functional relationships between genes. Here we describe a robust and scalable method to systematically map genetic interactions in human cancer cells using combinatorial RNAi and high-throughput imaging. Through automated, single-cell phenotyping, we measured genetic interactions across a broad spectrum of phenotypes, including cell count, cell eccentricity and nuclear area. We mapped genetic interactions of epigenetic regulators in colon cancer cells, recovering known protein complexes. Our study also revealed the prospects and challenges of studying genetic interactions in human cells using multiparametric phenotyping.
- Subjects
GENE mapping; CANCER cells; RNA interference; PHENOTYPES; EPIGENETICS; COLON cancer
- Publication
Nature Methods, 2013, Vol 10, Issue 5, p427
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.2436