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- Title
A photoactivatable marker protein for pulse-chase imaging with superresolution.
- Authors
Fuchs, Jochen; Böhme, Susan; Oswald, Franz; Hedde, Per Niklas; Krause, Maike; Wiedenmann, Jörg; Nienhaus, G. Ulrich
- Abstract
IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms. Here we introduce a monomeric variant, mIrisFP, and demonstrate how its multiple photoactivation modes can be used for pulse-chase experiments combined with subdiffraction-resolution imaging in living cells by using dual-color photoactivation localization microscopy (PALM).
- Subjects
CELLS; PROTEINS; HIGH resolution imaging; IRRADIATION; FLUORESCENCE microscopy
- Publication
Nature Methods, 2010, Vol 7, Issue 8, p627
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1477