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- Title
Digital transcriptome profiling using selective hexamer priming for cDNA synthesis.
- Authors
Armour, Christopher D.; Castle, John C.; Chen, Ronghua; Babak, Tomas; Loerch, Patrick; Jackson, Stuart; Shah, Jyoti K.; Dey, John; Rohl, Carol A.; Johnson, Jason M.; Raymond, Christopher K.
- Abstract
We developed a procedure for the preparation of whole transcriptome cDNA libraries depleted of ribosomal RNA from only 1 μg of total RNA. The method relies on a collection of short, computationally selected oligonucleotides, called 'not-so-random' (NSR) primers, to obtain full-length, strand-specific representation of nonribosomal RNA transcripts. In this study we validated the technique by profiling human whole brain and universal human reference RNA using ultra-high-throughput sequencing.
- Subjects
RIBOSOMAL DNA; OLIGONUCLEOTIDES; RNA physiology; DNA primers; BRAIN; HUMAN experimentation
- Publication
Nature Methods, 2009, Vol 6, Issue 9, p647
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1360