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- Title
Effect of Hydrogen Peroxide on Antibacterial Activities of Canadian Honeys.
- Authors
Brudzynski, K.
- Abstract
BACKGROUND Honey becomes increasingly recognized as a preventive medicinal agent, promoting health and well-being. In order to endorse the therapeutic aspect of honey, quality control criteria are required to measure honey efficacy and relate it to honey specific biomarkers. AIM The aim of this study was to determine whether hydrogen peroxide could serve as a biomarker for antibacterial activity of Canadian honeys. METHODS A total of 42 honey samples originating from different plant sources were analyzed using a broth microdilution assay against two bacterial strains: E. coli (ATCC 14948) and B. subtilis (ATCC 6633). The dose-response relationship between antibacterial activity and honey concentration for each bacterial strain were analyzed and MIC90 and MIC50 values were established. Each honey sample was further investigated for the production of hydrogen peroxide using the hydrogen peroxide/ peroxidase assay and the results were compared with levels of antibacterial activities of honey, expressed as MIC90. MAIN RESULTS The majority of honeys demonstrated moderate to high antibacterial activity with MIC90 ranging from 25% to 6.25% v/v. MIC90 and MIC50 values revealed that Canadian honeys exhibit a selective growth inhibitory activity against gramnegative E.coli. The endogenous formation of H2O2 varied among honey samples and ranged from 0.92 uM to 10.3 uM. The comparison of the total H2O2 concentrations with honeys' antibacterial activities expressed as MIC90 values showed no correlation between these two factors (linear correlation for E. coli: r = 0.15, p = 0.66 n.s. and B. subtilis: r = 0.039, p = 0.91 n.s.). CONCLUSIONS These data indicate that all Canadian honeys exhibited antibacterial activity, with higher selectivity against E.coli than B. subtilis, and that these antibacterial activities were not correlated with hydrogen peroxide production in honeys. Hydrogen peroxide levels in honey, therefore, are not predictive of the honeys' antibacterial activity.
- Subjects
HYDROGEN peroxide; ANTIBACTERIAL agents; HONEY; BIOMARKERS; ESCHERICHIA coli; BACILLUS subtilis
- Publication
Malaysian Journal of Medical Sciences, 2007, Vol 14, Issue 1, p108
- ISSN
1394-195X
- Publication type
Article