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- Title
STANDARDIZATION AND REPRODUCIBILITY OF RANDOM MARKER BASED ANALYSIS OF MICROPROPAGATED CRIMSON BEEBALM.
- Authors
ŽIAROVSKÁ, Jana; BEŽO, Milan; HRDLIČKOVÁ, Michaela; FERNANDÉZ, Eloy
- Abstract
Genetic analysis of the stability in micropropagated plant material are required to be perform by effective and reproducible techniques. When no specific DNA based markers are available for certain species, such as for crimson beebalm, standardized and optimized protocols can be used even for random based markers. Here we report for the first time the optimization of PCR conditions for RAPD analysis for the purposes of the establishment of fast, reliable and reproducible method for screening of genetic stability in Monarda dydima, L. micropropagated cultures. RAPD protocol was optimized based on the use of 50 or 100 ng of template DNA and annealing temperature of 36°C, resulted optimal amplification. Setting the standardized PCR conditions, primers used in experiments generated reproducible and distinguishable fingerprinting patterns for micropropagated cultures of crimson beebalm and is suitable for further analysis of studying genetic variation of this medicinal plant.
- Subjects
PLANT micropropagation; GENETIC markers in plants; BOTANICAL specimens; RAPD technique; PLANT species; POLYMERASE chain reaction
- Publication
Genetika (0534-0012), 2014, Vol 46, Issue 3, p855
- ISSN
0534-0012
- Publication type
Article
- DOI
10.2298/GENSR1403855Z