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- Title
Impact of transduction towards the proliferation and migration as well as the transduction efficiency of human umbilical cord-derived late endothelial progenitor cells with nine recombinant adeno-associated virus serotypes.
- Authors
Wang, Zhaofei; Fu, Qiang; Cao, Jianguo; Deng, Xiyun; Li, Yue; Wang, Qiaofeng; Zheng, Zhaofen
- Abstract
Objectives: To evaluate the transduction efficiency of human umbilical cord-derived, late endothelial progenitor cells late (HUCB-late EPCs) with nine recombinant adeno-associated virus (rAAV) serotypes and the ability of proliferation and migration of the cells after transduction. Results: rAAV2 and rAAV6 showed a greater ability than other serotypes to transduce late EPCs (P < 0.05). After transduction, cell proliferation ability weakened (P < 0.05), but the ability of migration to stromal cell-derived factor (SDF-1) unchanged. Conclusion: There is an advantage of choosing the optimal rAAV serotype as a gene vector to alter the biologic characteristics of late EPCs.
- Subjects
ENDOTHELIAL cells; SEROTYPES; GENETIC transduction; CELL proliferation; STROMAL cells
- Publication
Biotechnology Letters, 2016, Vol 38, Issue 7, p1073
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-016-2082-7