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- Title
Characterization and high expression of recombinant Ustilago maydis xylanase in Pichia pastoris.
- Authors
Han, Hongjuan; You, Shuang; Zhu, Bo; Fu, Xiaoyan; Sun, Baihui; Qiu, Jin; Yu, Chengye; Chen, Lei; Peng, Rihe; Yao, Quanhong
- Abstract
A recombinant xylanase gene ( rxynUMB) from Ustilago maydis 521 was expressed in Pichia pastoris, and the enzyme was purified and characterized. Phylogenetic analysis demonstrated that rxynUMB belongs to glycosyl hydrolase family 11. The Trp84, Trp95, Glu93, and Glu189 residues are proposed to be present at the active site. The apparent molecular mass of the recombinant xylananse was approximately 24 kDa, and the optimum pH and temperature were 4.3 and 50 °C, respectively. Xylanase activity was enhanced by 166 and 115 % with Fe and Mn, respectively. The biochemical properties of this recombinant xylanase suggest that it may be a useful candidate for a variety of commercial applications.
- Subjects
XYLANASES; RECOMBINANT proteins; USTILAGO maydis; PICHIA pastoris; GENE expression; CLADISTIC analysis; MICROORGANISMS
- Publication
Biotechnology Letters, 2015, Vol 37, Issue 3, p697
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-014-1716-x