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- Title
Enhanced transesterification of ethyl ferulate with glycerol for preparing glyceryl diferulate using a lipase in ionic liquids as reaction medium.
- Authors
Sun, Shangde; Qin, Fei; Bi, Yanlan; Chen, Jingnan; Yang, Guolong; Liu, Wei
- Abstract
Glyceryl diferulate (DFG) is a water-soluble ester of ferulic acid. A novel ionic liquid (IL) system for enzymatic transesterification of ethyl ferulate (EF) with glycerol to produce DFG was developed. Of three ILs with different anions (BF, PF and TFN) and cations (BDMIM, OMIM, HMIM, BMIM, and EMIM), EMIMTFN proved the best using a commercial lipase. It had a significant protective effect against thermal inactivation of the enzyme. High EF conversion (~100 %) and DFG yield (45 %) were achieved using 45 mg enzyme/ml; temperature, 70 °C; reaction time, 12 h.
- Subjects
TRANSESTERIFICATION; GLYCERIN; IONIC liquids; FUSED salts; LIPASES
- Publication
Biotechnology Letters, 2013, Vol 35, Issue 9, p1449
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-013-1222-6