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- Title
Rosette Nanotubes Alter IgE-Mediated Degranulation in the Rat Basophilic Leukemia (RBL)-2H3 Cell Line.
- Authors
Ede, James D.; Ortega, Van A.; Boyle, David; Beingessner, Rachel L.; Hemraz, Usha D.; Fennin, Hicham; Stafford, James L.; Goss, Greg G.
- Abstract
In this study, the effects of rosette nanotube (RNT) exposure on immune cell viability and function was investigated in vitro using the rat basophilic leukemia (RBL)-2H3 cell line. RBL-2H3 viability was decreased in a dose- and time-dependent manner after lysine-functionalized RNT (K-RNT) exposure. In addition, K-RNTs had a significant effect on RBL-2H3 degranulation. When K-RNT exposure was concurrent with IgE sensitization, 50 and 100 mg L-1 K-RNTs elicited a heightened degranulatory response compared to IgE alone. Exposure to 50 and 100 mg L-1 K-RNTs also caused degranulation in RBL-2H3 cells not sensitized with IgE (0 ng mL-1 IgE). Furthermore, in cells pre-exposed to K-RNTs for 2 h and subsequently washed, sensitized, and stimulated with IgE, a potentiated degranulatory response was observed. Using confocal laser scanning microscopy and a FITC-functionalized RNT construct (termed FITC¹/TBL19-RNT), we demonstrated a strong and direct affiliation between RNTs and RBL-2H3 cell membranes. We also demonstrated cellular internalization of RNTs after 2 h of exposure. Together, these data demonstrate that RNTs may affiliate with the cellular membrane of RBL-2H3 cells and can be internalized. These interactions can affect viability and alter the ability of these cells to elicit IgE-FcεR mediated degranulation.
- Subjects
LEUKEMIA immunology; NANOTUBES; IMMUNOGLOBULIN E; CELL survival; IMMUNE response; LABORATORY rats
- Publication
Toxicological Sciences, 2015, Vol 148, Issue 1, p108
- ISSN
1096-6080
- Publication type
Article
- DOI
10.1093/toxsci/kfv166