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- Title
Transcriptional Changes during Phytophthora capsici Infection Reveal Potential Defense Mechanisms in Squash.
- Authors
Acharya, Shailesh Raj; Shrestha, Swati; Michael, Vincent Njung'e; Fu, Yuqing; Sabharwal, Prerna; Thakur, Shallu; Meru, Geoffrey
- Abstract
Phytophthora capsici incites foliar blight, root, fruit, and crown rot in squash (Cucurbita spp.) and limits production worldwide. Resistance to crown rot in C. moschata breeding line #394-1-27-12 is conferred by three dominant genes, but the molecular mechanisms underlying this resistance are poorly understood. In the current study, RNA sequencing was used to investigate transcriptional changes in #394-1-27-12 (resistant) and Butterbush (susceptible) following infection by P. capsici at 12, 24, 48, 72, and 120 h post inoculation (hpi). Overall, the number of differentially expressed genes (DEGs) in Butterbush (2648) exceeded those in #394-1-27-12 (1729), but in both genotypes, the highest number of DEGs was observed at 72 hpi and least at 24 hpi. Our gene ontology (GO) analysis revealed a downregulation of the genes involved in polysaccharide and lignin metabolism in Butterbush but as an upregulation of those associated with regulation of peptidase activity. However, in #394-1-27-12, the downregulated genes were primarily associated with response to stimuli, whereas those upregulated were involved in oxidation–reduction and response to stress. The upregulated genes in #394-1-27-12 included defensin-like proteins, respiratory-burst oxidases, ethylene-responsive transcription factors, cytochrome P450 proteins, and peroxidases. These findings provide a framework for the functional validation of the molecular mechanisms underlying resistance to P. capsici in cucurbits.
- Subjects
PHYTOPHTHORA capsici; SQUASHES; PEPTIDASE; RNA sequencing; PEROXIDASE
- Publication
Stresses, 2023, Vol 3, Issue 4, p827
- ISSN
2673-7140
- Publication type
Article
- DOI
10.3390/stresses3040056