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- Title
MOLECULAR CHARACTERIZATION AND IN VITRO PROPAGATION BEHAVIOR OF POPULUS ALBA L. PLANTS UNDER EFFECT OF SLOW GROWTH CONSERVATION.
- Authors
Salim, Rasha G.; El-Sayed, Iman M.; Taha, Lobna S.
- Abstract
This work was aimed to identify the optimum osmotic stress condition that needed for in vitro short- term preservation protocol and detection of genetic variation of micropropagated Populus alba L. plants. The experiment was carried out to examine the micropropagation ability of Populus alba L. shootlets under the effect of different strengths of culture medium, sucrose and sorbitol (as osmotic stress was firstly used with Populus alba L. plants concentrations as slow growth treatments during various conservation periods (2, 4 and 6 months) to asses an in vitro protocol for conservation of the genetic diversity of the species. For maintaining survived shootlets (100%) for 4 months of conservation period, full or half strength of culture medium enriched with 30g/l sucrose could be used. Replacing sucrose by sorbitol (30 g/l) added to half strength of MS led to the highest percent of rooting after 6 months conservation. In vitro shootlet regrowth ability were obtained with full or half MS containing sucrose 30g/l followed by half MS containing sorbitol 30g/l. The highest rooting percent and roots number were recorded for in vitro resurvived shootlets which were cultured on half strength MS supplemented with sucrose 30g/l for 6 months conservation. Based on ISSR and SCoT marker analysis, the highest percentage value of similarity (81%) was between the shootlets cultured on half strength of MS medium supplemented with 30g/l sucrose and those cultured in the same medium supplemented with 30g/l sorbitol. The data obtained from this study revealed that ISSR and SCoT molecular markers have been proved to be more accurate in genetic diversity studies because of their high reproducibility and great power for the detection of polymorphism.
- Subjects
PLANT growth; PLANT micropropagation; SORBITOL; POPULUS alba; PLANT conservation
- Publication
Plant Archives (09725210), 2019, Vol 19, Issue 2, p2443
- ISSN
0972-5210
- Publication type
Article