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- Title
Effect of neurotrophins on differentiation, calcification and proliferation in cultures of human pulp cells
- Authors
Mizuno, Noriyoshi; Shiba, Hideki; Xu, Wan-peng; Inui, Takafumi; Fujita, Tsuyoshi; Kajiya, Mikihito; Takeda, Katsuhiro; Hasegawa, Naohiko; Kawaguchi, Hiroyuki; Kurihara, Hidemi
- Abstract
Abstract: Neurotrophins (NTs) are expressed during tooth development. However, little is known about a role of NTs in differentiation of pulp cells into mineralizing cells. In this study, mRNA expressions of hard tissue-related proteins, calcification and proliferation are examined in cultures of human pulp (HP) cells. Nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), neurotrophin (NT)-3 and NT-4/5 increased the mRNA levels of dentin sialophsphoprotein, alkaline phosphatase, osteopontin, type I collagen and bone morphogenetic protein-2 and mineral deposition in cultures of HP cells. The increased levels and manners varied, depending on the concentrations of NTs and hard-tissue related protein tested. On the other hand, only NGF significantly stimulated DNA synthesis in cultures of HP cells. These findings suggest that NTs characteristically regulate hard-tissue related protein expression, calcification and proliferation in pulp cells. NTs may accelerate pulp cell differentiation.
- Subjects
CELLS; OVUM; BIOMINERALIZATION; CALCIUM in the body
- Publication
Cell Biology International, 2007, Vol 31, Issue 12, p1462
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1016/j.cellbi.2007.06.012