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- Title
Genome Analysis of Vallitalea guaymasensis Strain L81 Isolated from a Deep-Sea Hydrothermal Vent System.
- Authors
Schouw, Anders; Vulcano, Francesca; Roalkvam, Irene; Hocking, William Peter; Stokke, Runar; Steen, Ida Helene; Reeves, Eoghan; Bødtker, Gunhild
- Abstract
Abyssivirga alkaniphila strain L81T, recently isolated from a black smoker biofilm at the Loki's Castle hydrothermal vent field, was previously described as a mesophilic, obligately anaerobic heterotroph able to ferment carbohydrates, peptides, and aliphatic hydrocarbons. The strain was classified as a new genus within the family Lachnospiraceae. Herein, its genome is analyzed and A. alkaniphila is reassigned to the genus Vallitalea as a new strain of V. guaymasensis, designated V. guaymasensis strain L81. The 6.4 Mbp genome contained 5651 protein encoding genes, whereof 4043 were given a functional prediction. Pathways for fermentation of mono-saccharides, di-saccharides, peptides, and amino acids were identified whereas a complete pathway for the fermentation of n-alkanes was not found. Growth on carbohydrates and proteinous compounds supported methane production in co-cultures with Methanoplanus limicola. Multiple confurcating hydrogen-producing hydrogenases, a putative bifurcating electron-transferring flavoprotein—butyryl-CoA dehydrogenase complex, and a Rnf-complex form a basis for the observed hydrogen-production and a putative reverse electron-transport in V. guaymasensis strain L81. Combined with the observation that n-alkanes did not support growth in co-cultures with M. limicola, it seemed more plausible that the previously observed degradation patterns of crude-oil in strain L81 are explained by unspecific activation and may represent a detoxification mechanism, representing an interesting ecological function. Genes encoding a capacity for polyketide synthesis, prophages, and resistance to antibiotics shows interactions with the co-occurring microorganisms. This study enlightens the function of the fermentative microorganisms from hydrothermal vents systems and adds valuable information on the bioprospecting potential emerging in deep-sea hydrothermal systems.
- Subjects
MOHNS Ridge; HYDROTHERMAL vent microbiology; CHEMOSYNTHESIS (Biochemistry); HETEROTROPHIC bacteria; BIOFILMS; BACTEROIDETES
- Publication
Microorganisms, 2018, Vol 6, Issue 3, p63
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms6030063