We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Repeated batch fermentation for surfactin production with immobilized Bacillus subtilis BS-37: two-stage pH control and foam fractionation.
- Authors
Yi, Gaobin; Liu, Qiang; Lin, Junzhang; Wang, Weidong; Huang, He; Li, Shuang
- Abstract
BACKGROUND Surfactin, a lipopeptide biosurfactant produced by Bacillus strains, is one of the most surface-active biosurfactants, and has the potential to enhance oil recovery or environmental remediation. However, low yield and high production costs limit its use in high-volume applications. In this work, Bacillus subtilis BS-37 was immobilized on a piece of cotton towel in a 5 L stirred tank reactor; the fill-and-draw process and foam fractionation were used for repeated batch fermentations. A two-stage pH control operation was developed for surfactin fermentation: for repeated batch fermentation, the pH value was 5.0 for the biomass accumulation in the previous 6 h; and then the pH value was raised to 7.5 for surfactin production over the following 18 h of fermentation. RESULTS The immobilized Bacillus subtilis BS-37 cells were efficiently reused for 7 cycles, producing 7887.3 mg surfactin in total. CONCLUSIONS The repeated batch fermentation, low-cost foam fractionation, reusable immobilized cells, and energy-efficient open (nonsterile) fermentation could greatly promote the development of industrial production of surfactin. © 2016 Society of Chemical Industry
- Subjects
SURFACTIN; BACILLUS subtilis biotechnology; FOAM fractionation (Sewage purification); BIOMASS production; ENCAPSULATION (Catalysis); ENHANCED oil recovery; ENVIRONMENTAL remediation
- Publication
Journal of Chemical Technology & Biotechnology, 2017, Vol 92, Issue 3, p530
- ISSN
0268-2575
- Publication type
Article
- DOI
10.1002/jctb.5028