We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A Synthetic Pan-Aurora Kinase Inhibitor, 5-Methoxy-2-(2-methoxynaphthalen-1-yl)-4H-chromen-4-one, Triggers Reactive Oxygen Species-Mediated Apoptosis in HCT116 Colon Cancer Cells.
- Authors
Lee, Jeong Yeon; Ahn, Sung Shin; Jeong, You Jeong; Choi, Jihye; Ahn, Seunghyun; Koh, Dongsoo; Lee, Young Han; Lim, Yoongho; Shin, Soon Young
- Abstract
Aurora kinases are Ser/Thr kinases that function as mitotic regulators. 5-Methoxy-2-(2-methoxynaphthalen-1-yl)-4H-chromen-4-one (DK1913) is a synthetic pan-Aurora kinase inhibitor. However, the mode of action of DK1913 concerning the induction of apoptosis is unclear. Here, we report that DK1913 triggered apoptosis, as revealed by flow cytometry and Annexin V staining. DK1913 enhanced the intracellular levels of reactive oxygen species (ROS) and stimulated the endoplasmic reticulum (ER) and genotoxic stress responses. We also found that DK1913 induced the loss of mitochondrial membrane potential, leading to the activation of caspase-9, caspase-7, and caspase-3. In addition, the antioxidant, butylated hydroxyanisole (BHA), abrogated DK1913-induced loss of mitochondrial membrane potential and activation of the caspase cascade. These findings demonstrate that pan-Aurora kinase inhibitor DK1913 triggers apoptosis through ROS-mediated ER and genotoxic stress responses.
- Subjects
CANCER cells; KINASE inhibitors; REACTIVE oxygen species; COLON cancer; AURORA kinases; ENDOPLASMIC reticulum
- Publication
Journal of Chemistry, 2020, p1
- ISSN
2090-9063
- Publication type
Article
- DOI
10.1155/2020/3025281